detectSpatialCorFeatsMatrix#

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Last Updated: Mar 11, 2023

Description#

Detect genes that are spatially correlated

Usage#

detectSpatialCorFeatsMatrix(
  expression_matrix,
  method = c("grid", "network"),
  spatial_network,
  spatial_grid,
  spatial_locs,
  subset_feats = NULL,
  network_smoothing = NULL,
  min_cells_per_grid = 4,
  cor_method = c("pearson", "kendall", "spearman")
)

Arguments#

expression_matrix

provided expression matrix

method

method to use for spatial averaging

spatial_network

provided spatial network

spatial_grid

provided spatial grid

spatial_locs

provided spatial locations

subset_feats

subset of features to use

network_smoothing

smoothing factor beteen 0 and 1 (default: automatic)

min_cells_per_grid

minimum number of cells to consider a grid

cor_method

correlation method

Details#

For method = network, it expects a fully connected spatial network. You can make sure to create a fully connected network by setting minimal_k > 0 in the createSpatialNetwork function.

  • 1. grid-averaging: average gene expression values within a predefined spatial grid

  • 2. network-averaging: smoothens the gene expression matrix by averaging the expression within one cell by using the neighbours within the predefined spatial network. b is a smoothening factor that defaults to 1 - 1/k, where k is the median number of k-neighbors in the selected spatial network. Setting b = 0 means no smoothing and b = 1 means no contribution from its own expression.

The spatCorObject can be further explored with showSpatialCorGenes()

Value#

returns a spatial correlation object: “spatCorObject”

See Also#

showSpatialCorFeats